Phytoestrogens regulate mRNA and protein levels of guanine nucleotide‐binding protein, beta‐1 subunit (GNB1) in MCF‐7 cells
Identifieur interne : 002870 ( Main/Exploration ); précédent : 002869; suivant : 002871Phytoestrogens regulate mRNA and protein levels of guanine nucleotide‐binding protein, beta‐1 subunit (GNB1) in MCF‐7 cells
Auteurs : Srivatcha Naragoni [États-Unis] ; Shireesha Sankella [États-Unis] ; Kinesha Harris [États-Unis] ; Wesley G. Gray [États-Unis]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2009-06.
English descriptors
- Teeft :
- Amersham pharmacia, Assay, Beta, Biochem, Bioinformatics analysis, Biol, Biological effects, Breast cancer, Cdna, Cdna library, Cellular physiology, Chip assay, Chromatin, Clone, Differential product, Differential products, Differentially, Easy vector, Endocrinol, Estradiol, Estrogen, Estrogen receptor, Estrogenic, Estrogenic effects, Gapdh, Gene, Gene expression, Genistein, Gnb1, Gnb1 cdna, Gnb1 expression, Gnb1 gene, Gnb1 mrna, Gnb1 mrna expression, Gnb1 promoter, Gnb1 protein expression, Gnb1 protein levels, Half sites, Hybridization, Hybridization buffer, Hybridized, Immunoprecipitated, Kinase, Ligand, Loading control, Membrane, Mrna, Northern blot analysis, Online issue, Pathway, Pharmacol, Physiology, Phytoestrogen, Phytoestrogens, Primer, Promoter, Promoter region, Prostate, Prostate cancer, Protein expression, Protein levels, Putative, Real time, Receptor, Responsive genes, Room temperature, Signal transduction, Software, Steroid, Steroid biochem, Subtractive, Subtractive hybridization, Subunit, Suppression subtractive hybridization, Target genes, Tester, Total protein, Transcription, Transcription factors, Transcriptional, Transcriptional activation, Transduction, Treatment control, Western blot, Western blot analysis.
Abstract
Phytoestrogens (PEs) are non‐steroidal ligands, which regulate the expression of number of estrogen receptor‐dependent genes responsible for a variety of biological processes. Deciphering the molecular mechanism of action of these compounds is of great importance because it would increase our understanding of the role(s) these bioactive chemicals play in prevention and treatment of estrogen‐based diseases. In this study, we applied suppression subtractive hybridization (SSH) to identify genes that are regulated by PEs through either the classic nuclear‐based estrogen receptor or membrane‐based estrogen receptor pathways. SSH, using mRNA from genistein (GE) treated MCF‐7 cells as testers, resulted in a significant increase in GNB1 mRNA expression levels as compared with 10 nM 17β estradiol or the no treatment control. GNB1 mRNA expression was up regulated two‐ to fivefold following exposure to 100.0 nM GE. Similarly, GNB1 protein expression was up regulated 12‐ to 14‐fold. GE regulation of GNB1 was estrogen receptor‐dependent, in the presence of the anti‐estrogen ICI‐182,780, both GNB1 mRNA and protein expression were inhibited. Analysis of the GNB1 promoter using ChIP assay showed a PE‐dependent association of estrogen receptor α (ERα) and β (ERβ) to the GNB1 promoter. This association was specific for ERα since association was not observed when the cells were co‐incubated with GE and the ERα antagonist, ICI. Our data demonstrate that the levels of G‐protein, beta‐1 subunit are regulated by PEs through an estrogen receptor pathway and further suggest that PEs may control the ratio of α‐subunit to β/γ‐subunits of the G‐protein complex in cells. J. Cell. Physiol. 219: 584–594, 2009. © 2009 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jcp.21699
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002396
- to stream Istex, to step Curation: 002396
- to stream Istex, to step Checkpoint: 000667
- to stream Main, to step Merge: 002896
- to stream Main, to step Curation: 002870
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phytoestrogens regulate mRNA and protein levels of guanine nucleotide‐binding protein, beta‐1 subunit (GNB1) in MCF‐7 cells</title><author><name sortKey="Naragoni, Srivatcha" sort="Naragoni, Srivatcha" uniqKey="Naragoni S" first="Srivatcha" last="Naragoni">Srivatcha Naragoni</name></author><author><name sortKey="Sankella, Shireesha" sort="Sankella, Shireesha" uniqKey="Sankella S" first="Shireesha" last="Sankella">Shireesha Sankella</name></author><author><name sortKey="Harris, Kinesha" sort="Harris, Kinesha" uniqKey="Harris K" first="Kinesha" last="Harris">Kinesha Harris</name></author><author><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:78CD2B6A944AAD0E0EE4B821352F40169195C737</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1002/jcp.21699</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-W3JK82J8-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002396</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002396</idno><idno type="wicri:Area/Istex/Curation">002396</idno><idno type="wicri:Area/Istex/Checkpoint">000667</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000667</idno><idno type="wicri:doubleKey">0021-9541:2009:Naragoni S:phytoestrogens:regulate:mrna</idno><idno type="wicri:Area/Main/Merge">002896</idno><idno type="wicri:Area/Main/Curation">002870</idno><idno type="wicri:Area/Main/Exploration">002870</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Phytoestrogens regulate mRNA and protein levels of guanine nucleotide‐binding protein, beta‐1 subunit (GNB1) in MCF‐7 cells</title><author><name sortKey="Naragoni, Srivatcha" sort="Naragoni, Srivatcha" uniqKey="Naragoni S" first="Srivatcha" last="Naragoni">Srivatcha Naragoni</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Environmental Toxicology, Southern University and A&M College, Baton Rouge</wicri:cityArea></affiliation></author><author><name sortKey="Sankella, Shireesha" sort="Sankella, Shireesha" uniqKey="Sankella S" first="Shireesha" last="Sankella">Shireesha Sankella</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Environmental Toxicology, Southern University and A&M College, Baton Rouge</wicri:cityArea></affiliation></author><author><name sortKey="Harris, Kinesha" sort="Harris, Kinesha" uniqKey="Harris K" first="Kinesha" last="Harris">Kinesha Harris</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Chemistry, Southern University and A&M College, Baton Rouge</wicri:cityArea></affiliation></author><author><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Environmental Toxicology, Southern University and A&M College, Baton Rouge</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Department of Chemistry, Southern University and A&M College, Baton Rouge</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Louisiane</region></placeName><wicri:cityArea>Correspondence address: Department of Environmental Toxicology, 116 Lee Hall, P.O. Box 9716, Southern University‐Baton Rouge, Baton Rouge</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Cellular Physiology</title><title level="j" type="alt">JOURNAL OF CELLULAR PHYSIOLOGY</title><idno type="ISSN">0021-9541</idno><idno type="eISSN">1097-4652</idno><imprint><biblScope unit="vol">219</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="584">584</biblScope><biblScope unit="page" to="594">594</biblScope><biblScope unit="page-count">11</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2009-06">2009-06</date></imprint><idno type="ISSN">0021-9541</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9541</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amersham pharmacia</term><term>Assay</term><term>Beta</term><term>Biochem</term><term>Bioinformatics analysis</term><term>Biol</term><term>Biological effects</term><term>Breast cancer</term><term>Cdna</term><term>Cdna library</term><term>Cellular physiology</term><term>Chip assay</term><term>Chromatin</term><term>Clone</term><term>Differential product</term><term>Differential products</term><term>Differentially</term><term>Easy vector</term><term>Endocrinol</term><term>Estradiol</term><term>Estrogen</term><term>Estrogen receptor</term><term>Estrogenic</term><term>Estrogenic effects</term><term>Gapdh</term><term>Gene</term><term>Gene expression</term><term>Genistein</term><term>Gnb1</term><term>Gnb1 cdna</term><term>Gnb1 expression</term><term>Gnb1 gene</term><term>Gnb1 mrna</term><term>Gnb1 mrna expression</term><term>Gnb1 promoter</term><term>Gnb1 protein expression</term><term>Gnb1 protein levels</term><term>Half sites</term><term>Hybridization</term><term>Hybridization buffer</term><term>Hybridized</term><term>Immunoprecipitated</term><term>Kinase</term><term>Ligand</term><term>Loading control</term><term>Membrane</term><term>Mrna</term><term>Northern blot analysis</term><term>Online issue</term><term>Pathway</term><term>Pharmacol</term><term>Physiology</term><term>Phytoestrogen</term><term>Phytoestrogens</term><term>Primer</term><term>Promoter</term><term>Promoter region</term><term>Prostate</term><term>Prostate cancer</term><term>Protein expression</term><term>Protein levels</term><term>Putative</term><term>Real time</term><term>Receptor</term><term>Responsive genes</term><term>Room temperature</term><term>Signal transduction</term><term>Software</term><term>Steroid</term><term>Steroid biochem</term><term>Subtractive</term><term>Subtractive hybridization</term><term>Subunit</term><term>Suppression subtractive hybridization</term><term>Target genes</term><term>Tester</term><term>Total protein</term><term>Transcription</term><term>Transcription factors</term><term>Transcriptional</term><term>Transcriptional activation</term><term>Transduction</term><term>Treatment control</term><term>Western blot</term><term>Western blot analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytoestrogens (PEs) are non‐steroidal ligands, which regulate the expression of number of estrogen receptor‐dependent genes responsible for a variety of biological processes. Deciphering the molecular mechanism of action of these compounds is of great importance because it would increase our understanding of the role(s) these bioactive chemicals play in prevention and treatment of estrogen‐based diseases. In this study, we applied suppression subtractive hybridization (SSH) to identify genes that are regulated by PEs through either the classic nuclear‐based estrogen receptor or membrane‐based estrogen receptor pathways. SSH, using mRNA from genistein (GE) treated MCF‐7 cells as testers, resulted in a significant increase in GNB1 mRNA expression levels as compared with 10 nM 17β estradiol or the no treatment control. GNB1 mRNA expression was up regulated two‐ to fivefold following exposure to 100.0 nM GE. Similarly, GNB1 protein expression was up regulated 12‐ to 14‐fold. GE regulation of GNB1 was estrogen receptor‐dependent, in the presence of the anti‐estrogen ICI‐182,780, both GNB1 mRNA and protein expression were inhibited. Analysis of the GNB1 promoter using ChIP assay showed a PE‐dependent association of estrogen receptor α (ERα) and β (ERβ) to the GNB1 promoter. This association was specific for ERα since association was not observed when the cells were co‐incubated with GE and the ERα antagonist, ICI. Our data demonstrate that the levels of G‐protein, beta‐1 subunit are regulated by PEs through an estrogen receptor pathway and further suggest that PEs may control the ratio of α‐subunit to β/γ‐subunits of the G‐protein complex in cells. J. Cell. Physiol. 219: 584–594, 2009. © 2009 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Louisiane</li></region></list><tree><country name="États-Unis"><region name="Louisiane"><name sortKey="Naragoni, Srivatcha" sort="Naragoni, Srivatcha" uniqKey="Naragoni S" first="Srivatcha" last="Naragoni">Srivatcha Naragoni</name></region><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name><name sortKey="Gray, Wesley G" sort="Gray, Wesley G" uniqKey="Gray W" first="Wesley G." last="Gray">Wesley G. Gray</name><name sortKey="Harris, Kinesha" sort="Harris, Kinesha" uniqKey="Harris K" first="Kinesha" last="Harris">Kinesha Harris</name><name sortKey="Sankella, Shireesha" sort="Sankella, Shireesha" uniqKey="Sankella S" first="Shireesha" last="Sankella">Shireesha Sankella</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002870 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002870 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:78CD2B6A944AAD0E0EE4B821352F40169195C737
|texte= Phytoestrogens regulate mRNA and protein levels of guanine nucleotide‐binding protein, beta‐1 subunit (GNB1) in MCF‐7 cells
}}
| This area was generated with Dilib version V0.6.33. |  |